Concurrent Metabolic Profiling and Quantification of Aromatic Amino Acids and Phytohormones in Solanum lycopersicum Plants Responding to Phytophthora capsici.
Identifieur interne : 000263 ( Main/Exploration ); précédent : 000262; suivant : 000264Concurrent Metabolic Profiling and Quantification of Aromatic Amino Acids and Phytohormones in Solanum lycopersicum Plants Responding to Phytophthora capsici.
Auteurs : Msizi I. Mhlongo [Afrique du Sud] ; Lizelle A. Piater [Afrique du Sud] ; Paul A. Steenkamp [Afrique du Sud] ; Nico Labuschagne [Afrique du Sud] ; Ian A. Dubery [Afrique du Sud]Source :
- Metabolites [ 2218-1989 ] ; 2020.
Abstract
Pathogenic microorganisms account for large production losses in the agricultural sector. Phytophthora capsici is an oomycete that causes blight and fruit rot in important crops, especially those in the Solanaceae family. P. capsici infection is difficult to control due to genetic diversity, arising from sexual reproduction, and resistant spores that remain dormant in soil. In this study, the metabolomics of tomato plants responding to infection by P. capsici were investigated. Non-targeted metabolomics, based on liquid chromatography coupled to mass spectrometry (LC-MS), were used with multivariate data analyses to investigate time-dependent metabolic reprogramming in the roots, stems, and leaves of stem-infected plants, over an 8 day period. In addition, phytohormones and amino acids were determined using quantitative LC-MS. Methyl salicylate and 1-aminocyclopropane-1-carboxylate were detected as major signalling molecules in the defensive response to P. capsici. As aromatic amino acid precursors of secondary metabolic pathways, both phenylalanine and tryptophan showed a continuous increase over time in all tissues, whereas tyrosine peaked at day 4. Non-targeted metabolomic analysis revealed phenylpropanoids, benzoic acids, glycoalkaloids, flavonoids, amino acids, organic acids, and fatty acids as the major classes of reprogrammed metabolites. Correlation analysis showed that metabolites derived from the same pathway, or synthesised by different pathways, could either have a positive or negative correlation. Furthermore, roots, stems, and leaves showed contrasting time-dependent metabolic reprogramming, possibly related to the biotrophic vs. necrotrophic life-stages of the pathogen, and overlapping biotic and abiotic stress signaling. As such, the targeted and untargeted approaches complemented each other, to provide a detailed view of key time-dependent metabolic changes, occurring in both the asymptomatic and symptomatic stages of infection.
DOI: 10.3390/metabo10110466
PubMed: 33207638
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Mhlongo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006, South Africa.</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>Department of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006</wicri:regionArea><wicri:noRegion>Johannesburg 2006</wicri:noRegion></affiliation></author><author><name sortKey="Piater, Lizelle A" sort="Piater, Lizelle A" uniqKey="Piater L" first="Lizelle A" last="Piater">Lizelle A. Piater</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006, South Africa.</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>Department of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006</wicri:regionArea><wicri:noRegion>Johannesburg 2006</wicri:noRegion></affiliation></author><author><name sortKey="Steenkamp, Paul A" sort="Steenkamp, Paul A" uniqKey="Steenkamp P" first="Paul A" last="Steenkamp">Paul A. Steenkamp</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006, South Africa.</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>Department of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006</wicri:regionArea><wicri:noRegion>Johannesburg 2006</wicri:noRegion></affiliation></author><author><name sortKey="Labuschagne, Nico" sort="Labuschagne, Nico" uniqKey="Labuschagne N" first="Nico" last="Labuschagne">Nico Labuschagne</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant and Soil Sciences, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa.</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>Department of Plant and Soil Sciences, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028</wicri:regionArea><wicri:noRegion>Pretoria 0028</wicri:noRegion></affiliation></author><author><name sortKey="Dubery, Ian A" sort="Dubery, Ian A" uniqKey="Dubery I" first="Ian A" last="Dubery">Ian A. Dubery</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006, South Africa.</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>Department of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006</wicri:regionArea><wicri:noRegion>Johannesburg 2006</wicri:noRegion></affiliation></author></analytic><series><title level="j">Metabolites</title><idno type="ISSN">2218-1989</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pathogenic microorganisms account for large production losses in the agricultural sector. <i>Phytophthora capsici</i> is an oomycete that causes blight and fruit rot in important crops, especially those in the Solanaceae family. <i>P. capsici</i> infection is difficult to control due to genetic diversity, arising from sexual reproduction, and resistant spores that remain dormant in soil. In this study, the metabolomics of tomato plants responding to infection by <i>P. capsici</i> were investigated. Non-targeted metabolomics, based on liquid chromatography coupled to mass spectrometry (LC-MS), were used with multivariate data analyses to investigate time-dependent metabolic reprogramming in the roots, stems, and leaves of stem-infected plants, over an 8 day period. In addition, phytohormones and amino acids were determined using quantitative LC-MS. Methyl salicylate and 1-aminocyclopropane-1-carboxylate were detected as major signalling molecules in the defensive response to <i>P. capsici</i>. As aromatic amino acid precursors of secondary metabolic pathways, both phenylalanine and tryptophan showed a continuous increase over time in all tissues, whereas tyrosine peaked at day 4. Non-targeted metabolomic analysis revealed phenylpropanoids, benzoic acids, glycoalkaloids, flavonoids, amino acids, organic acids, and fatty acids as the major classes of reprogrammed metabolites. Correlation analysis showed that metabolites derived from the same pathway, or synthesised by different pathways, could either have a positive or negative correlation. Furthermore, roots, stems, and leaves showed contrasting time-dependent metabolic reprogramming, possibly related to the biotrophic vs. necrotrophic life-stages of the pathogen, and overlapping biotic and abiotic stress signaling. As such, the targeted and untargeted approaches complemented each other, to provide a detailed view of key time-dependent metabolic changes, occurring in both the asymptomatic and symptomatic stages of infection.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">33207638</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2218-1989</ISSN><JournalIssue CitedMedium="Print"><Volume>10</Volume><Issue>11</Issue><PubDate><Year>2020</Year><Month>Nov</Month><Day>16</Day></PubDate></JournalIssue><Title>Metabolites</Title><ISOAbbreviation>Metabolites</ISOAbbreviation></Journal><ArticleTitle>Concurrent Metabolic Profiling and Quantification of Aromatic Amino Acids and Phytohormones in <i>Solanum lycopersicum</i> Plants Responding to <i>Phytophthora capsici</i>.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E466</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/metabo10110466</ELocationID><Abstract><AbstractText>Pathogenic microorganisms account for large production losses in the agricultural sector. <i>Phytophthora capsici</i> is an oomycete that causes blight and fruit rot in important crops, especially those in the Solanaceae family. <i>P. capsici</i> infection is difficult to control due to genetic diversity, arising from sexual reproduction, and resistant spores that remain dormant in soil. In this study, the metabolomics of tomato plants responding to infection by <i>P. capsici</i> were investigated. Non-targeted metabolomics, based on liquid chromatography coupled to mass spectrometry (LC-MS), were used with multivariate data analyses to investigate time-dependent metabolic reprogramming in the roots, stems, and leaves of stem-infected plants, over an 8 day period. In addition, phytohormones and amino acids were determined using quantitative LC-MS. Methyl salicylate and 1-aminocyclopropane-1-carboxylate were detected as major signalling molecules in the defensive response to <i>P. capsici</i>. As aromatic amino acid precursors of secondary metabolic pathways, both phenylalanine and tryptophan showed a continuous increase over time in all tissues, whereas tyrosine peaked at day 4. Non-targeted metabolomic analysis revealed phenylpropanoids, benzoic acids, glycoalkaloids, flavonoids, amino acids, organic acids, and fatty acids as the major classes of reprogrammed metabolites. Correlation analysis showed that metabolites derived from the same pathway, or synthesised by different pathways, could either have a positive or negative correlation. Furthermore, roots, stems, and leaves showed contrasting time-dependent metabolic reprogramming, possibly related to the biotrophic vs. necrotrophic life-stages of the pathogen, and overlapping biotic and abiotic stress signaling. As such, the targeted and untargeted approaches complemented each other, to provide a detailed view of key time-dependent metabolic changes, occurring in both the asymptomatic and symptomatic stages of infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mhlongo</LastName><ForeName>Msizi I</ForeName><Initials>MI</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006, South Africa.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Piater</LastName><ForeName>Lizelle A</ForeName><Initials>LA</Initials><Identifier Source="ORCID">0000-0002-9526-0109</Identifier><AffiliationInfo><Affiliation>Department of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006, South Africa.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Steenkamp</LastName><ForeName>Paul A</ForeName><Initials>PA</Initials><Identifier Source="ORCID">0000-0002-4416-6796</Identifier><AffiliationInfo><Affiliation>Department of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006, South Africa.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Labuschagne</LastName><ForeName>Nico</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Plant and Soil Sciences, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dubery</LastName><ForeName>Ian A</ForeName><Initials>IA</Initials><Identifier Source="ORCID">0000-0002-9079-4769</Identifier><AffiliationInfo><Affiliation>Department of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006, South Africa.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Metabolites</MedlineTA><NlmUniqueID>101578790</NlmUniqueID><ISSNLinking>2218-1989</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Phytophthora capsici</Keyword><Keyword MajorTopicYN="N">aromatic amino acids</Keyword><Keyword MajorTopicYN="N">correlation</Keyword><Keyword MajorTopicYN="N">metabolomics</Keyword><Keyword MajorTopicYN="N">phytohormones</Keyword><Keyword MajorTopicYN="N">time-dependent metabolic reprogramming</Keyword><Keyword MajorTopicYN="N">tomato</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>10</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>11</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>11</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>19</Day><Hour>1</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>20</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33207638</ArticleId><ArticleId IdType="pii">metabo10110466</ArticleId><ArticleId IdType="doi">10.3390/metabo10110466</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Afrique du Sud</li></country></list><tree><country name="Afrique du Sud"><noRegion><name sortKey="Mhlongo, Msizi I" sort="Mhlongo, Msizi I" uniqKey="Mhlongo M" first="Msizi I" last="Mhlongo">Msizi I. Mhlongo</name></noRegion><name sortKey="Dubery, Ian A" sort="Dubery, Ian A" uniqKey="Dubery I" first="Ian A" last="Dubery">Ian A. Dubery</name><name sortKey="Labuschagne, Nico" sort="Labuschagne, Nico" uniqKey="Labuschagne N" first="Nico" last="Labuschagne">Nico Labuschagne</name><name sortKey="Piater, Lizelle A" sort="Piater, Lizelle A" uniqKey="Piater L" first="Lizelle A" last="Piater">Lizelle A. Piater</name><name sortKey="Steenkamp, Paul A" sort="Steenkamp, Paul A" uniqKey="Steenkamp P" first="Paul A" last="Steenkamp">Paul A. Steenkamp</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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